安定類藥物ELISA快速檢測試劑盒(檢組織/尿樣/飼料,靈敏度0.1ppb,兩步法)
安定類藥物ELISA快速檢測試劑盒
一、原理
本試劑盒采用間接競爭ELISA方法,在微孔條上預(yù)包被偶聯(lián)抗原,樣本中的殘留物安定類藥物將和微孔條上預(yù)包被的偶聯(lián)抗原競爭抗安定類藥物抗體,加入酶標(biāo)記物后,用TMB底物顯色,樣本吸光值與其所含殘留物安定類藥物的含量成負(fù)相關(guān),與標(biāo)準(zhǔn)曲線比較即可得出相應(yīng)殘留物安定類藥物的含量。
二、試劑盒特性
u 試劑盒規(guī)格 : 96孔/盒;
u 試劑盒靈敏度: 0.1ppb
u 曲線范圍: 0.1~8.1ppb
u 孵育溫度: 37℃
u 孵育時間: 30min~30min~15min
u 試劑盒貯存條件: 2~8℃;
u 板內(nèi)變異系數(shù): <5%;
u 板間變異系數(shù): <15%;
u 樣本檢測下限
組 織 ········································· 1ppb
尿 液 ········································· 1ppb
飼 料 ········································ 10ppb
u 交叉反應(yīng)率
地+#西泮 ············································ 100%
硝西+#泮 ············································ 7.6%
奧沙西 ············································ 8.8%
u 樣本回收率
飼 料 ········································· 75±20%
組 織 ········································· 85±19%
尿 液 ········································· 80±17%
三、試劑盒組成
1 | 微量測試孔 | 每條8孔,一板12條 | |
2 | 標(biāo)準(zhǔn)液×6瓶 (1ml/瓶) | 0ppb | 0.1ppb |
0.3ppb | 0.9ppb | ||
2.7ppb | 8.1ppb | ||
3 | 酶標(biāo)記物 | 12ml | 紅色帽 |
4 | 抗體工作液 | 7ml | 藍(lán)色帽 |
5 | 底物A液 | 7ml | 白色帽 |
6 | 底物B液 | 7ml | 黑色帽 |
7 | 終止液 | 7ml | 黃色帽 |
8 | 20X濃縮洗滌液 | 40ml | 白色帽 |
9 | 2X濃縮復(fù)溶液 | 50ml | 透明帽 |
四、樣品前處理步驟及檢測分析步驟
詳見產(chǎn)品說明書。
五、儲藏條件和保質(zhì)期
1、 儲藏條件:試劑盒于2-8℃保存,不要冷凍。
2、 保 質(zhì) 期:該產(chǎn)品有效期為1年,生產(chǎn)日期見包裝盒。
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